Journal: The American Journal of Pathology

Article Title: MDM2

doi: 10.2353/ajpath.2006.051351

Figure Lengend Snippet: A: Representative RT-PCR analysis of total RNA from cultured human VSMCs (HASMC). RNA was amplified in the presence of oligonucleotide primers specific for MR (top panel) and 11β-HSD type 2 (bottom panel). Extracts from human kidney were used as a positive control (P). B: Representative immunoblotting studies demonstrating MR and 11β-HSD type 2 proteins in HASMC. Extracts from human kidney were used as a positive control (P). C and D: Immunocytochemical analysis demonstrated that immunopositive cells for MR appear brown as a result of diaminobenzidine colorimetric reaction in HASMC without ligand stimulation (C) or treated with 10 nmol/L aldosterone (D) (Original magnification, ×400).

Article Snippet: Cell Culture and Characterization A cultured human VSMC cell line, ie, HASMC (derived from human abdominal aorta) was commercially obtained from Kurabo Corp. (Osaka, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Cell Culture, Amplification, Positive Control, Western Blot

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH induces HASMC apoptosis. Apoptosis was detected by flow cytometry. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. Percentages of PS-positive/PI-negative and PS-positive/PI-positive cells are shown. (D) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Flow Cytometry, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH increases cleaved caspase 3 and caspase 12 expression as detected by Western blot analysis. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days. (B, E) Data are the mean ± SD of three independent experiments. (D) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. (C, F) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Expressing, Western Blot, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH increases Bax, Bad, and Bim expression and decreases Bcl-2 expression as detected by RT-PCR. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days.(B) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH activates the PERK-CHOP ER stress pathway as detected by Western blot analysis. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. (D) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Western Blot, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH activates the IRE1-JNK ER stress pathway as detected by Western blot analysis. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. (D) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Western Blot, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH increases GRP78/BiP expression as detected by Western blot analysis. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. (D) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Expressing, Western Blot, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: Inhibition of JNK or CHOP blocks PTH-induced HASMC apoptosis. Apoptosis was detected by flow cytometry. (A) HASMCs were pretreated with SP6000125, a JNK inhibitor, and then treated with 1 × 10 −6 mol/L PTH for 3 days. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were transfected with si-CHOP and then treated with 1 × 10 −6 mol/L PTH for three days. (D) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus PTH group.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Inhibition, Flow Cytometry, Transfection, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: Inhibition of JNK or CHOP suppresses PTH-induced cleaved caspase 3 expression as detected by Western blot analysis. (A) HASMCs were pretreated with SP6000125, a JNK inhibitor, and then treated with 1 × 10 −6 mol/L PTH for three days. HASMCs cultured in normal medium for the same days as control. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were transfected with si-CHOP and then treated with 1 × 10 −6 mol/L PTH for three days. HASMCs cultured in scramble siRNA for the same days as control. (D) Data are the mean ± SD of three independent experiments. * p < .05 versus PTH group.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Inhibition, Expressing, Western Blot, Cell Culture, Control, Transfection